Thomas nhs foundation hospitals trust, london, england semin thromb hemost 2014. New or revised interpretive criteria are available for the following drugs. M100s25 is available in print and electronic pdf formats in tandem with m02a12, m07a10, or both documents. For further information on committee participation or to submit comments, contact clsi. Statistical quality control for quantitative measurement procedures. With these readonly web versions of m100 and m60, you can now quickly reference the most trusted ast and antifungal breakpoints from anywhere with an internet connection. Clsi clinical laboratory standards institute m100s26. For these three speciesdrug combinations, susceptible clinical inhibition zone diameter breakpoints differ substantially between eucast 1. Footnotes based on clsi document m100s26 a oxacillinresistant staphylococci are resistant to all other betalactam class of agents, i.
It is important for clinical laboratories to incorporate the new recommendations into routine practice to optimize detection and reporting of antimicrobial resistance. Development of in vitro susceptibility testing criteria and quality control parameters third edition. What version of the clsi m100 document is currently being used by your microbiology laboratory circle one. The data in the interpretive tables in this supplement are valid only if the. Impact of revised penicillin breakpoints for streptococcus pneumoniae clsi m100s18 on the penicillin susceptibility rate. Clsi m100s24 performance standards for antimicrobial susceptibility testing. Clinical and laboratory standards institute, 950 west valley road, suite 2500, wayne, pennsylvania 19087 usa, 2014. Performance standards for antimicrobial susceptability testing clsi.
Jan 29, 2009 clsi, formerly nccls, is a global, nonprofit, membershipbased organization dedicated to developing standards and guidelines for the health care and medical testing community. Impact assessment of revised clsi cephalosporin breakpoints. The complete standardmay be obtainedfrom the clinical and. If you required documents prior to 2008, contact cqi programs office. Change of antibiotic susceptibility testing guidelines from. The clinical and laboratory standards institute clsi has published a revised document titled methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria m45ed3. Clinical and laboratory standards institute, 940 west valley road, suite 1400, wayne, pennsylvania 19087 usa, 2011. Performance standards for antimicrobial susceptibility testing. Comparison of agar dilution and broth microdilution methods. This document provides updated tables for the clinical and laboratory standards institute antimicrobial susceptibility testing standards m02a11, m07a9, and m11.
Gsk2140944 is a novel bacterial type ii topoisomerase inhibitor in development for the treatment of conventional and biothreat pathogens, including grampositive pathogens and methicillinresistant staphylococcus aureus. M02a11 isbn 1562387812 print isbn 1562387820 electronic issn 15586502 print volume 32 number 1 issn 21622914 electronic performance standards for antimicrobial disk susceptibility. When testing a patients isolate, a clinical laboratory can either use one of the clsi reference methods i. This guideline informs clinical, public health, and research laboratories on susceptibility testing of infrequently isolated or fastidious bacteria. Disc di ffusion supplemental tables performance standards for antimicrobial susceptibility testing. Twofold dilutions of clindamycin, moxifloxacin and metronidazole and threefold dilutions of vancomycin were defined as acceptable mic ranges according to the quality control ranges for c. Clsi publishes 2009 antimicrobial susceptibility testing. Product code title approval date status bd vacutainer order of draw for multiple tube collections illustration. Clsi publishes a revised microbiology document lablogatory.
In january 2017, the annual update of the m100 tables clsi document m100 performance standards for antimicrobial susceptibility testing, 27th edition will publish. Includes the m02 quick guide this disk diffusion reading guide provides photographic examples of the proper method for reading disk diffusion susceptibility testing results. Each year, the clsi updates the m100s document with recommendations for agents to testreport, interpretive criteria, and qc ranges. The tetracyclines were the initial group or class of broadspectrum antimicrobials to be described, dating from 1944, when chlortetracycline was isolated from streptomyces aureofaciens 5, 12. This document provides updated tables for the clinical and laboratory standards institute antimicrobial susceptibility testing standards m02a11, m07a9, and m11a8. Revised clsi breakpoints 2010 for s showed greater. Clinical and laboratory standards institute 950 west valley road, suite 2500 wayne, pa 19087 usa p. Clsi a one health perspective on susceptibility testing. Documents available janet hindler discussion slide handout janet hindler m100 s19, m02a10, and m07a8 checklist if you have budgetary issues purchasing m100 s19, please call nphl or me 402 559. Synthesis, structure and sar of pyranbased lpxc inhibitors. Each january, clsi updates standards for antimicrobial susceptibility testing ast.
Jan 06, 2017 clinical and laboratory standards institute january 27 at 11. These revised clsi criteria result in slightly decreased s rates for cro 3. Clinical and laboratory standards institute, 950 west valley road, suite 2500, wayne, pennsylvania 19087 usa, 2018. This standard covers the current recommended methods for disk susceptibility testing and criteria for quality control testing. Determination of disk diffusion and mic quality control. Through our partnerships program, clsi provides international outreach services and handson support to laboratories in resourceconstrained countries, helping them achieve sustainable quality with systems to better diagnose and treat patients with infectious diseases. An online implementation of m100s25 called em100 is also available. Clsi m100 s20 breakpoints were reduced most for cro 8x and to a lesser extent for atm 2x and caz 2x, but unchanged for cpm. Clsi m100s24 performance standards for antimicrobial. Clinical and laboratory standards institute january 27 at 11.
Recent guidelines and recommendations for laboratory. Available online as a convenient companion to our m100 document and m60 document. In january 2015, the annual update of the m100 tables clsi document m100s25 will publish. Clsi m02ed and m100ed29 package performance standards for antimicrobial disk susceptibility tests, thirteenth edition, m02ede. Issn 15586502 print replaces m100 s24 issn 21622914 electronic vol. Recent guidelines and recommendations for laboratory detection of lupus anticoagulants gary w. Clsiaphl 2017 antimicrobial susceptibility testing. Documents available janet hindler discussion slide handout janet hindler m100s19, m02a10, and m07a8 checklist if you have budgetary issues purchasing m100s19, please call nphl or me 402 559. This document includes updated tables for the clinical and laboratory standards institute antimicrobial susceptibility testing standards m02, m07, and m11. Some highlights for 2017 include additional suggestions for testing colistinpolymyxin b and poorly growing staphylococcus aureus. Reevaluation of clinical and laboratory standards institute. What version of the clsi m100 document was used by your microbiology laboratory. Performance standards for antimicrobial susceptibility.
Clsi m100s20 breakpoints were reduced most for cro 8x and to a lesser extent for atm 2x and caz 2x, but unchanged for cpm. Clsi, formerly nccls, is a global, nonprofit, membershipbased organization dedicated to developing standards and guidelines for the health care and medical testing community. In january 2015, the annual update of the m100 tables clsi document m100 s25 will publish. Approved guideline, c24a3 clinical and laboratory standards institute on. Listed modifications were recently approved by the clsi m100s17.
Mar 14, 2007 listed modifications were recently approved by the clsi m100s17. Clsi m45 methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria. The complete standardmay be obtainedfrom the clinical and laboratory standards institute, 940 west valley road, suite 1400, wayne, pa19807. The page below is a sample from the labce course 2014 updates and recommendations from the clsi for antimicrobial susceptibility testing and reporting. Clsi documents on file in cqi office the clsi documents listed on these pages have approval dates beginning from 2008 to 2014. Each test included reference compounds and quality control reference strains to validate both the compound preparation and experimental methodologies used. Performance standards for antimicrobial susceptibility testing, twentyninth edition, m100ed29e. Antimicrobial susceptibility testing standards, 415 cap today. The complete standardmay be obtainedfrom the clinical and laboratory standards institute, 940.